Systematic Evaluation of O-glycosylation Mediated Enhancement and Modulation of the Cel7A Carbohydrate Binding Module Function
نویسنده
چکیده
.........................................................................................................................................3 Introduction....................................................................................................................................4 i. Crystalline Cellulose Affinity Enhancement by O-‐glycosylation of Cel7A CBM .................6 ii. O-‐glycan Modulation of Cel7A CBM Cellulose Specificity....................................................9 iii. Lignin Non-‐productive Adsorption by Cel7A CBM and Modulation by O-‐glycosylation.12 Materials and Methods......................................................................................................................14 Results and Discussion................................................................................................................20 i. Synthesis and Characterization of CBM Glycoforms.................................................20 ii. Cel7A CBM O-‐Mannosylation Confers Adsorption Enhancement Towards Crystalline Cellulose....................................................................................................20 iii. O-‐Mannose Modulation of Cel7A CBM Specificity for Model Cellulose Systems........26 iv. CBM Glycoform Non-‐productive Adsorption on Kraft Lignin...................................30 Concluding Remarks and Future Directions.............................................................................33 Acknowledgements.....................................................................................................................35 References.....................................................................................................................................36 Appendix I.....................................................................................................................................48 Appendix II...................................................................................................................................49
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تاریخ انتشار 2015